Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Growth hormone-releasing hormone agonists ameliorate chronic kidney disease-induced heart failure with preserved ejection fraction

doi: 10.1073/pnas.2019835118

Figure Lengend Snippet: Titin and pro-BNP expression in myocardium. (A) Expression and phosphorylation of titin isoforms. Representative image of 1% agarose gel electrophoresis with SYPRO Ruby and ProQ Diamond phosphoprotein stain for titin analysis of LV. Bar graphs show relative hypophosphorylation of both N2BA and N2B isoforms, as well as the N2BA/N2B ratio in the control (white; n = 3), placebo (orange; n = 6), and GHRH- A (blue; n = 6) groups. The relative phosphorylated titin expression is the average of triplicate values. All data are expressed as mean ± SEM. *P < 0.05 by Kruskal–Wallis test. (B) Expression of pro-BNP in the myocardium. Representative Western blot for pro-BNP expression from swine myocardial biopsies from control (lanes 1 to 3 and 16 to 18), placebo-treated (lanes 4 to 6 and 10 to 12), and GHRH-A–treated (lanes 7 to 9 and 13 to 15). Quantification of Western blots for all samples; control (n = 3), placebo treated (n = 6), and GHRH-A treated (n = 6). **P = 0.003 placebo vs. GHRH-A by Dunn’s multiple-comparisons test.

Article Snippet: The gels were stained for 60 min with ProQ Diamond (Molecular Probes).

Techniques: Expressing, Agarose Gel Electrophoresis, Staining, Western Blot